Epithalon 10mg

Important Note

This page is informational and does not authorize use. Peptify clients should complete assessment, disclose medications and health history, and follow the clinician-approved plan only.

Do not start, stop, combine, or change a protocol based only on website content.
Emergency symptoms require urgent medical care, not a website or routine follow-up message.

Quickstart Highlights

Epithalon (also written Epitalon) is a synthetic tetrapeptide — sequence Ala-Glu-Asp-Gly — modelled on the pineal-gland peptide epithalamin[1][2]. It has been studied — largely in older Russian (Khavinson) research — for telomerase activation, telomere maintenance and circadian/melatonin regulation. This educational page outlines a short subcutaneous cycle with a dilution chosen so doses land on easy-to-read insulin-syringe marks. Epithalon is not approved by the FDA, EMA or any major Western regulator; the evidence is mostly older and preclinical with limited independent replication. It is an unapproved research chemical, presented for research and educational use only.

Add 2.0 mL bacteriostatic water to one 10 mg vial → 5 mg/mL (5,000 mcg/mL), a clean dilution that lands doses on round insulin-syringe marks.
5–10 mg once daily for a short ~10–20 day cycle, typically repeated 2–3× per year.
At 5 mg/mL, 1 unit = 50 mcg; 5 mg = 100 units (1.0 mL) and 10 mg = 200 units (2.0 mL) on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.

Dosing & Reconstitution Guide

A single practical dilution with accurate once-daily dosing, step by step

Standard Cycle Approach (2 mL = 5 mg/mL)

Cycle Day	Dose & Frequency	Volume (U-100 units / mL)
Days 1–5	5 mg / 5000 mcg (1× daily)	100 units (1.00 mL)
Days 6–10	5 mg / 5000 mcg (1× daily)	100 units (1.00 mL)
Days 11–20 (optional extension)	5–10 mg / 5000–10000 mcg (1× daily)	100–200 units (1.00–2.00 mL)

Reconstitute: Add 2.0 mL bacteriostatic water to one 10 mg vial → final concentration 5 mg/mL (5,000 mcg/mL).
Typical dose: 5–10 mg once daily over a short ~10–20 day cycle, repeated 2–3× per year.
Easy measuring: At 5 mg/mL, 1 unit = 50 mcg on a U-100 syringe, so 5 mg = 100 units and 10 mg = 200 units — whole, easy-to-read volumes.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Frequency: one subcutaneous injection each day for a short cycle of roughly 10–20 days, commonly repeated 2–3 times per year in cited research schedules[3][4]. These figures come from older reference protocols, not from approved human dosing.

Reconstitution Steps

Draw 2.0 mL of bacteriostatic water into a sterile syringe.

Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 2.0 mL dilution gives a clean 5 mg/mL concentration, so a 5 mg dose reads at exactly 100 units (1.0 mL) on a U-100 syringe. Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptide.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Quantities below assume a short 10–20 day cycle of once-daily injections, repeated a few times per year.

A 10 mg vial covers 1–2 days at 5–10 mg/day, so a single ~10–20 day cycle needs several vials.
10-day cycle (5 mg/day): ~5 vials
20-day cycle (5 mg/day): ~10 vials
20-day cycle (10 mg/day): ~20 vials
Per injection: 1 syringe
10-day cycle: ~10 syringes
20-day cycle: ~20 syringes
Use ~2.0 mL per 10 mg vial for reconstitution.
10-day cycle (5 vials): ~10 mL → 1 bottle
20-day cycle (10 vials): ~20 mL → 2 bottles
One for the vial stopper + one for the injection site each day.
Per injection: 2 swabs
20-day cycle (once daily): ~40 swabs → 1 box

Protocol Overview

A concise summary of the short cycled regimen, drawn from older cited reference protocols.

▪Goal: Studied for telomerase activation, telomere maintenance and circadian/melatonin regulation — effects reported mostly in older Russian and preclinical work, not established in humans[5][6].
▪Schedule: Daily subcutaneous injections for a short ~10–20 day cycle, typically repeated 2–3 times per year.
▪Dose Range: 5–10 mg per day for the duration of the short cycle.
▪Reconstitution: 2.0 mL bacteriostatic water per 10 mg vial gives 5 mg/mL for accurate unit measurements.
▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.

Dosing Protocol

A suggested short-cycle approach based on older reference doses.

▪Start: Begin at 5 mg once daily to gauge tolerability.
▪Hold or extend: Maintain 5 mg/day, or extend toward 10 mg/day for the optional days 11–20 window if tolerated.
▪Target: A cumulative cycle dose in the range of roughly 50–100 mg across the 10–20 day course.
▪Cycle Length: Typically ~10–20 days, repeated 2–3 times per year.
▪Timing: Inject at a consistent time each day and rotate injection sites systematically.

Storage Instructions

Correct storage is what preserves the peptide’s stability and activity.

▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days; do not freeze the mixed solution, as freezing can denature peptides[8].
▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.

Important Notes

Practical points that keep daily administration safe and consistent.

▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
▪Regulatory note: Epithalon is not approved by the FDA, EMA or any major Western regulator for human use, and its evidence base is limited and largely unreplicated outside older Russian research[10].

How This Works

Epithalon (Epitalon) is a synthetic tetrapeptide with the sequence Ala-Glu-Asp-Gly (AEDG), designed as a short analogue of the natural pineal-gland peptide epithalamin[1][2]. The pineal peptides it is modelled on are involved in circadian and neuroendocrine regulation.

Its most-discussed proposed mechanism is telomerase activation — the enzyme that maintains telomeres at the ends of chromosomes. In cell-culture and animal work from the Khavinson group, Epithalon has been reported to induce telomerase expression, support telomere maintenance, and influence gene expression[5][6]. These findings come largely from older Russian research.
It has also been studied for circadian and melatonin regulation — restoring more youthful melatonin rhythms in aged animals — and, in long-term rodent studies, for effects framed as longevity / anti-aging[11]. Such longevity claims should be read cautiously as hypotheses, not established outcomes.
Important caveat: the human evidence base is thin and largely unreplicated outside the original Russian groups, and most data are preclinical or older clinical work without modern independent confirmation[13]. Anti-aging and telomere claims rest on this limited literature and should not be overstated.
Epithalon is not approved by the FDA, EMA or any major Western regulator. It is an unapproved research chemical presented here for research and educational purposes only.

Lifestyle Factors

Habits that support general healthy aging alongside the protocol.

▪Nutrition: A balanced, antioxidant-rich diet supports general cellular health during a cycle.
▪Activity & rest: Pair regular activity with consistent recovery; chronic overtraining works against healthy aging.
▪Sleep: Aim for 7–9 hours; consistent sleep reinforces the circadian rhythms Epithalon is studied for.
▪Stress: Manage stress with evidence-based practices, since it influences overall cellular aging.

Potential Benefits & Side Effects

What older preclinical and Russian research describe; human evidence is limited and largely unreplicated.

▪Telomerase & telomeres (preclinical): Cell and animal studies report telomerase activation and telomere maintenance[5][6].
▪Circadian / melatonin (preclinical): Reported to help restore melatonin rhythms and circadian regulation in aged animals[11].
▪Longevity signals (preclinical): Some long-term rodent studies describe lifespan-related effects — framed as hypotheses, not proven benefits.
▪Note on humans: These benefits are not established in humans — the evidence is mostly older Russian and preclinical work with limited independent replication[13].
▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
▪Unapproved status: Epithalon is not approved by any major Western regulator; quality and long-term safety are not independently assured.

Injection Technique

General subcutaneous technique, following established clinical best-practice guidance[14][15].

▪Wash your hands well with soap and water.
▪Wipe the vial stopper with an alcohol swab and let it air-dry.
▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
▪Draw the intended dose, then check for air bubbles and push any out.
▪Pinch a skinfold at the chosen site between thumb and forefinger.
▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
▪Press the plunger slowly and steadily until it’s fully down.
▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
▪Return the reconstituted vial to the fridge right away.
▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
▪Watch the site for excess redness, swelling, or signs of infection.

Recommended Source

For high-purity research peptides, we point researchers to Prime Lab Peptides. Note: Epithalon is not currently listed there, so the link below points to their main catalog.

▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA. Open source
▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
Visit Prime Lab Peptides →

References

Reference-derived details for Epithalon 10mg.

Epithalon (10mg Vial) Dosage Protocol Open source
1 Khavinson V.Kh. et al. (peptide bioregulators) Foundational work describing short pineal peptides (epithalamin) and the synthetic AEDG tetrapeptide Epithalon. View Source ↗ Open source
2 Bulletin of Experimental Biology and Medicine Reports on the synthesis and biological activity of the Ala-Glu-Asp-Gly (Epitalon) tetrapeptide. View Source ↗ Open source
3 Neuro Endocrinology Letters Cited Russian clinical/experimental protocols describing short cycled courses of Epithalon. View Source ↗ Open source
4 Advances in Gerontology (Russian) Reference gerontology protocols and cycled dosing schedules for pineal peptide preparations. View Source ↗ Open source
5 Khavinson V.Kh., Bondarev I.E., et al. (telomerase) Cell-culture work reporting telomerase induction and telomere elongation by the AEDG peptide. View Source ↗ Open source
6 Biogerontology Studies on gene-expression and telomere-related effects attributed to Epithalon in model systems. View Source ↗ Open source
7 Peptide handling / lyophilized storage guidance General guidance on storing lyophilized research peptides cold, dry and dark. View Source ↗ Open source
8 Peptide reconstitution & stability On reconstituting peptides with bacteriostatic water and avoiding freeze-thaw of solutions. View Source ↗ Open source
9 Injection-site rotation guidance Clinical guidance on rotating subcutaneous injection sites to limit irritation and lipohypertrophy. View Source ↗ Open source
10 Regulatory status (FDA/EMA) Epithalon is not an approved drug with any major Western regulator; it is an unapproved research chemical. View Source ↗ Open source
11 Experimental Gerontology Rodent studies describing melatonin/circadian restoration and lifespan-related effects of pineal peptides. View Source ↗ Open source
12 Peptide pharmacology review Background on short peptide bioregulators and their proposed neuroendocrine mechanisms. View Source ↗ Open source
13 Independent replication / evidence appraisal Notes that Epithalon human data are limited and largely unreplicated outside the original Russian groups. View Source ↗ Open source
14 Centers for Disease Control and Prevention (CDC) Subcutaneous injection technique: angle, site and no-aspiration guidance. View Source ↗ Open source
15 Subcutaneous Injection Technique (Patient Education) How to administer a subcutaneous injection: clinical technique guidelines. View Source ↗ Open source
16 Prime Lab Peptides Research-peptide supplier main catalog — purity specifications and certificates of analysis (Epithalon not currently listed). View Source ↗ Open source

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